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MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in <t>MB49</t> cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.
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MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in <t>MB49</t> cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.
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murine bladder cancer cell line mb49 cvcl_7076  (Millipore)
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MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in <t>MB49</t> cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.
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paper n a mouse bladder cancer cell line mb49  (ATCC)
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MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in <t>MB49</t> cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.
Paper N A Mouse Bladder Cancer Cell Line Mb49, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mb49 cell line  (Shanghai Genechem Ltd)
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Shanghai Genechem Ltd mb49 cell line
MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in <t>MB49</t> cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.
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MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in MB49 cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.

Journal: Advanced Science

Article Title: MCUB Inhibits PRKN‐Dependent Mitophagic Degradation of PD‐L1 to Promote Immune Evasion in Bladder Cancer

doi: 10.1002/advs.202514764

Figure Lengend Snippet: MCUB promoted PD‐L1 expression and tumor progression in BCa. A) IHC staining images of MCUB and PD‐L1 in 10 BCa samples. B) Correlation analysis between MCUB and PD‐L1 IHC scores. C) Western blotting of PD‐L1 expression in MCUB knockdown (shMCUB) and overexpression (oeMCUB) UMUC3 cells. D) Representative tumor images and tumor weights from xenograft models following MCUB knockdown or overexpression in MB49 cells ( N = 6 per group). E) IHC staining images of PD‐L1 in subcutaneous tumors at 20× and 40× magnifications. Statistical analysis was performed using Student's t ‐test. * p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: The murine BCa cell line MB49 (RRID: CVCL_7076) was obtained from Procell Life Science & Technology Co., Ltd (Wuhan, China) with catalogue number CL‐0733.

Techniques: Expressing, Immunohistochemistry, Western Blot, Knockdown, Over Expression